Irene Pandino
Tutor: Giuseppe GRASSO
Irene Pandino obtained a Bachelor's degree in Chemistry (L-17) in the academic year 2019/2020 and a Master's degree in Chemical Sciences (Biomolecular Chemistry Curriculum, LM-54) in the academic year 2021/2022. She qualified to practice as a Professional Chemist (Section A) in 2022 and is currently attending the PhD program in Chemical Sciences at the University of Catania, conducting her research in collaboration with the IRCCS Fondazione Bietti where she is part of the “Translational Research and Proteomics Laboratory.” Over the years of study and research, she has acquired various skills in the field of protein–ligand and protein–protein molecular interactions of biomedical, biological, and biochemical interest, the study of metal–peptide and metal–protein complexes, and the study of enzymatic pathways using Multi-Parametric Surface Plasmon Resonance (MP-SPR), Fluorescence and UV-Vis Spectroscopy, microfluidics and nanofluidics, chromatography-coupled mass spectrometry (HPLC–MS), immunoprecipitation, gel electrophoresis (SDS-PAGE) and Western Blotting, and Real-Time PCR (RT-PCR). In particular, during her internship for the Master's degree, in Prof. Grasso's Lab, she contributed to the conception and development of a new method based on Surface Plasmon Resonance (SPR) for the determination of molecular diffusion coefficients. To date, her research focuses on the proteomic, biochemical, and molecular study of glaucoma and neurodegenerative diseases, in combination with studies of the dysregulation of biochemical pathways. Specifically, her research concentrates on the post-translational modifications of proteins of pathological interest.
[07.04.2025]
Thesis title: Investigating the translational relevance of ubiquitin conjugation in neurodegeneration by chemical-physical, proteomics and molecular approaches.
Keywords: Neurodegeneration, Proteomics, Ubiquitin, Inflammation, Mass-Spectrometry.
Abstract: Ubiquitination is a post-translational modification in which ubiquitin modulates protein fate and function. Through diGLY approaches (immunoaffinity enrichment of Ub peptides) in mass spectrometry, the repertoire of Ub-proteins in brain neurodegeneration and ocular pathologies (glaucoma) will be studied using biological samples such as PBMCs and aqueous humor. The aim is to identify Ub-peptides specific to these pathologies and synthesize them to study their biochemical and physical properties.