Analytical Chemistry II and Laboratory A - L
Academic Year 2023/2024 - Teacher: Giuseppe SPOTOExpected Learning Outcomes
The aim is to provide background information on instrumental analytical chemistry with specific reference to spectroscopy and electrochemistry.
Course Structure
Class and laboratory experiments.
Should teaching be carried out in mixed mode or remotely, changes with respect to previous statements may be introduced, in line with the outlined syllabus.
According to safety requirements linked to the pandemic, learning assessment may also be carried out online.
Required Prerequisites
Attendance of Lessons
Attendance is compulsory as specified in the degree course Regolamento didattico and in the Regolamento didattico di Ateneo (art. 24). These regulations also explain when a total or partial exemption from the obligation may be recognized.
Detailed Course Content
1. Spectroscopy
Electromagnetic radiation: photoelectric effect, diffraction, mathematical representation, electromegnetic spectrum, refraction, refraction index, Snell law, total internal reflection. Radiation-matter interaction: replection, transmission/adsorption, diffusion( Rayleigh, Mie, Raman). Polarization. Adsorption and emission of radiation. Atomic adsorption.
Molecular adsorption: vibration and rotation. Fluorescence and phosphorescence. Jablonski diagram.
2. UV-vis spectroscopy
Trasmittance, adsorbance, Beer’s law: limit, chemical deviations, instrumental deviations. Photometers and spectrophotometers. Spetrophotometers: single beam, double beams. Sources. Filters and monochromators. Prisms. Transducers.
3. Infrared spectroscopy
Armonic and anarmonic oscillators. Vibrational modes, Fourier transform. Michelson interferometer. Sources. Infrared spectra. Sample requirements. Diffuse reflectance. ATR. Ifrared microscopy.
Raman spectroscopy
Raman effect. Polarizability. Comparison with IR spectroscopy. Sources. Raman microscopy.
Electroanalytical methods. Potentiometry
X-ray Fluorescence.
Laser
Statistics.
Diodes. LED, Operational amplifiers.
Textbook Information
Skoog, Holler, Nieman, “Principles of Instrumental Analysis”, Saunders College publishing.
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | Spettroscopia | Skoog, Leary, “Chimica Analitica Strumentale”, Edises |
| 2 | Spettroscopia UV-vis | Skoog, Leary, “Chimica Analitica Strumentale”, Edises. |
| 3 | Spettroscopia infrarossa | Skoog, Leary, “Chimica Analitica Strumentale”, Edises. |
| 4 | Spettroscopia Raman | Skoog, Leary, “Chimica Analitica Strumentale”, Edises. |
| 5 | Metodi elettrochimici | Skoog, Leary, “Chimica Analitica Strumentale”, Edises. |
| 6 | Fluorescenza di Raggi X | Skoog, Leary, “Chimica Analitica Strumentale”, Edises. |
| 7 | Laser | Skoog, Leary, “Chimica Analitica Strumentale”, Edises. |
| 8 | Statistica | Maria Garetto, "STATISTICA Lezioni ed esercizi". QUADERNI DIDATTICI del Dipartimento di Matematica. Università di Torino. |
Learning Assessment
Learning Assessment Procedures
Oral interview and evaluation of reports on laboratory experiments.
- Oral interview: Discussion to ascertain the level of knowledge pursued by the student on the course topics and analytical techniques used during the laboratory experiments. The student's ability to present the theoretical contents with an appropriate scientific language and the critical capacity acquired in dealing with experimental analytical chemistry will also be verified;
-Laboratory reports: The student will have to provide a written paper describing the experimental procedure followed, the results obtained, and data elaboration. The verification of learning can also be carried out online, should the conditions require it.
Examples of frequently asked questions and / or exercises
-Operational amplifiers and main operating modes
-Distinction between energy and intensity of electromagnetic radiation
-Selection of light sources used for Raman spectroscopy
-How can the expression to calculate the confidence interval of a parameter of a population be obtained?