METHODS FOR THE STUDY OF BIOINORGANIC SYSTEMS
Academic Year 2023/2024 - Teacher: Graziella VECCHIOExpected Learning Outcomes
Knowledge and understanding:
Knowledge of techniques for the study of biomolecules and bioinorganic systems
Applied knowledge and understanding:
Ability to apply the acquired knowledge to interpret experimental data and propose biomolecule characterization techniques
Autonomy of judgment:
Ability to critically interpret the data obtained using the techniques discussed in the course.
Communication skills:
Acquire language properties in describing the principles and applications of the techniques learned.
Ability to learn:
Develop the necessary skills by participating in lessons and studying the material provided and deepen the knowledge acquired in the characterization of biomolecules.
Course Structure
In-room lectures and discussion.
Required Prerequisites
Attendance of Lessons
Detailed Course Content
- Circular dichroism spectroscopy. Physical basis of CD. Selection rules. Exciton coupling. Static and dynamic coupling. Octant rule. CD sepctra of biomolecules.
- XAS spectroscopy. Physical bases. Application for the study of metalloproteins.
- The physical basis of NMR spectroscopy. Nuclear Angular Moment and magnetic moment. Nuclei in a magnetic field.
- Basic Principles of the NMR experiment. The resonance condition. The pulse. Relaxation. Fourier transformation.
- The Chemical Shift. Nuclear shielding.
- Coupling. Spin-Spin coupling. Equivalent neighboring nuclei. Dependence of bond angle.
- Double resonance experiments. Simplification of spectra by selective spin decoupling. NOE. effect. Decoupling in 13C NMR spectra.
- NMR 2D. Basic Principles. COSY. TOCSY, ETCOR, NOESY e ROESY. 3D and 4D NMR. Exercises.
- Dynamic NMR. Exchange processes. DOSY.
- MRI. Basic Principles. Contrast agents used in the clinic.
- EPR. Physical bases. Application for the study of metalloproteins.
Textbook Information
The Basics of NMR by J.P. Hornak Hypertext based NMR course http://www.cis.rit.edu/htbooks/nmr/nmr-main.htm
Horst Friebolin Basic One- and Two-Dimensional NMR Spectroscopy Wiley.VCH
Alison Rodger, Bengt Nordén Circular Dichroism and Linear Dichroism Oxford University Press
Course Planning
Subjects | Text References | |
---|---|---|
1 | Horst Friebolin Basic One- and Two-Dimensional NMR Spectroscopy Wiley.VCH | |
2 | Horst Friebolin Basic One- and Two-Dimensional NMR Spectroscopy Wiley.VCH | |
3 | Horst Friebolin Basic One- and Two-Dimensional NMR Spectroscopy Wiley.VCH | |
4 | Horst Friebolin Basic One- and Two-Dimensional NMR Spectroscopy Wiley.VCH | |
5 | Horst Friebolin Basic One- and Two-Dimensional NMR Spectroscopy Wiley.VCH | |
6 | Horst Friebolin Basic One- and Two-Dimensional NMR Spectroscopy Wiley.VCH | |
7 | Horst Friebolin Basic One- and Two-Dimensional NMR Spectroscopy Wiley.VCH | |
8 | Material provided to the students | |
9 | Material provided to students | |
10 | Material provided to students | |
11 | Material provided to students |
Learning Assessment
Learning Assessment Procedures
Learning will be verified during the course through classroom exercises and through a final exam.
The final exam consists of a written test and an oral test.
The written test consists of mostly open-ended application exercises.
After passing the written test (with a grade of at least 18/30) you can take the oral test, which is based on clarification of the written test and other verification questions.
The final mark takes into account the marks attributed to all the tests.